HPLC works adhering to The fundamental basic principle of slender layer chromatography or column chromatography, in which it's got a stationary stage and also a mobile section. The cellular period flows in the stationary period and carries the elements on the combination with it.
The cellular stage’s stream amount is decided through the mixed speeds of The 2 pumps. By modifying the relative speeds of the two pumps, diverse binary cellular phases is usually geared up.
物質の濃度により光の通過する角度が変わることを利用した検出器。原理上グラジェント分析はできない（グラジェントによって移動相自体の屈折率が変化するため）。また、感度が低いのが欠点だが、大部分の物質に対して使用できる。
- 분석결과는 재현성이 우수하며, 특히 오토샘플러 등을 사용함으로써 보다 높은 재현성을 확보할 수 있어 생산성을 한층 더 향상시킬 수 있습니다.
In reversed-section HPLC the buy of elution is the opposite that in a standard-phase separation, with much more polar solutes eluting 1st. Raising the polarity on the cellular phase causes extended retention periods. Shorter retention periods require a cellular stage of reduce polarity.
What is the concentration of caffeine in a very sample if a 10-μL injection gives a peak space of 424195? The info in this problem comes from Kusch, P.
The detector monitors the eluent and generates a signal, that is normally in the form of the chromatogram, which is a graphical illustration of compound concentration after some time.
As it employs a loop injection, the precision get more info of an HPLC technique often is better than a GC technique. HPLC isn't restricted to risky analytes, which implies we could examine a broader array of compounds. Capillary GC columns, Conversely, have far more theoretical plates, and will individual a lot more complicated mixtures.
The data acquisition system controls the HPLC instrument and collects the signal in the detector. This information and facts is exhibited being a chromatogram, a graph showing peaks equivalent to the separated analytes.
A pump forces a solvent through a column underneath high pressures of approximately four hundred atmospheres. The column packing materials or adsorbent or stationary phase is often a granular content of sound particles including silica or polymers.
The HPLC column homes the stationary section, a critical element for separating analytes. Deciding on the right column is critical:
Immediately after inserting the sample in the sample reservoir the injection course of action is absolutely automated. The injector injects the sample into your constantly flowing cellular section stream that carries the sample for the HPLC column.
The sample injector introduces the sample get more info into the HPLC system. Precise and exact sample injection is crucial for acquiring reputable benefits.
The separation of the person parts while in the combination normally takes place from the stationary stage from the column. Rather than the glass column, it is ready in chrome steel.